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Protech Technology Enterprise mmlv reverse transcription kit
Mmlv Reverse Transcription Kit, supplied by Protech Technology Enterprise, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mmlv+reverse+transcription+kit/kit+mmlv+reverse+transcription/pmc13142123-78-7-11
Average 86 stars, based on 1 article reviews
mmlv reverse transcription kit - by Bioz Stars, 2026-09
86/100 stars

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Purification:

Article Title: Gold nanoparticle-loaded macrophages enhance radiotherapy via immune remodeling in oral cancer
Article Snippet: RAW 264.7 cells were seeded and treated with 100 ppm GNPs, 1 μg/mL LPS (#19661, Cayman Chemical, MI, USA) or 20 ng/mL IL-4 (#214-14, PeproTech, NJ, USA) for 6 and 24 h. LPS and IL-4 were used to induce M1-and M2-like macrophages, respectively. .. RNA was extracted from cell pellets using the FavorPrepTM RNA purification kit (FABRK 001, Favorgen Biotech Corp, Pingtung, Taiwan) and reverse transcribed into complementary DNA (cDNA) using the MMLV reverse transcription kit (Protech, Taipei, Taiwan). .. Gene expression was quantified using the Thermo StepOnePlusTM real-time PCR system (Thermo Fisher Scientific, MA, USA).

Reverse Transcription:

Article Title: Gold nanoparticle-loaded macrophages enhance radiotherapy via immune remodeling in oral cancer
Article Snippet: RAW 264.7 cells were seeded and treated with 100 ppm GNPs, 1 μg/mL LPS (#19661, Cayman Chemical, MI, USA) or 20 ng/mL IL-4 (#214-14, PeproTech, NJ, USA) for 6 and 24 h. LPS and IL-4 were used to induce M1-and M2-like macrophages, respectively. .. RNA was extracted from cell pellets using the FavorPrepTM RNA purification kit (FABRK 001, Favorgen Biotech Corp, Pingtung, Taiwan) and reverse transcribed into complementary DNA (cDNA) using the MMLV reverse transcription kit (Protech, Taipei, Taiwan). .. Gene expression was quantified using the Thermo StepOnePlusTM real-time PCR system (Thermo Fisher Scientific, MA, USA).

Article Title: Polystyrene nanoplastics disrupt the intestinal microenvironment by altering bacteria-host interactions through extracellular vesicle-delivered microRNAs.
Article Snippet: Total RNA was extracted from renal tissues and cells using TRIzol® (Ambion,Waltham,MA, USA) following themanufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 2μg of total RNA using the MMLV Reverse Transcription Kit (Protech, Taipei, Taiwan). .. Quantitative PCR (qPCR) was performed using the Fast SYBRTM Green Master Mix (4385612, Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to themanufacturer’s protocol. mRNA expression levels were analyzed using real-time qPCR (StepOne Real-Time PCR System, USA).

Article Title: Polystyrene nanoplastics disrupt the intestinal microenvironment by altering bacteria-host interactions through extracellular vesicle-delivered microRNAs
Article Snippet: Total RNA was extracted from renal tissues and cells using TRIzol® (Ambion, Waltham, MA, USA) following the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 2 μg of total RNA using the MMLV Reverse Transcription Kit (Protech, Taipei, Taiwan). .. Quantitative PCR (qPCR) was performed using the Fast SYBRTM Green Master Mix (4385612, Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol. mRNA expression levels were analyzed using real-time qPCR (StepOne Real-Time PCR System, USA).

Article Title: The phosphorylation of Pak1 by Erk1/2 to drive cell migration requires Arl4D acting as a scaffolding protein.
Article Snippet: RNA extraction and RT-PCR To determine the mouse Arl4A mRNA expression level in NIH3T3 and A-10 cells, total RNA was extracted using the GENEzol Pure Kit (GZXD200, Geneaid) and subjected to an in-column DNase I digestion procedure. .. 1 μg of total RNA was reverse transcribed (RT) using the MMLV Reverse Transcription Kit (PT-RT-KIT, PROTECH). .. Subsequently, 1 μL of the RT product was used to perform polymerase chain reactions (PCRs) using VeriFi Mix (PB10.43-02, PCRBIOSYSTEMS), with mouse Arl4A specific primers for 25 cycles and with GAPDH primers for 20 cycles.

Article Title: Chronic sleep deprivation is associated with delayed puberty onset in rats, activation of proinflammatory cytokines and gut dysbiosis
Article Snippet: The RNA was extracted by using the RNeasy Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocol. .. Following RNA isolation, one μg of RNA was used for reverse transcription to cDNA by using the MMLV Reverse Transcription Kit (Protech Technology Enterprise, Co., Ltd., Taipei, Taiwan). cDNA was used to quantify the transcript levels on the Smart Quant Green Master Mix system (Protech Technology Enterprise, Co., Ltd., Taipei, Taiwan). ..

Article Title: Antiviral effect of lactoferrin against infectious bronchitis virus (IBV) are associated with alterations in the gastrointestinal microbiome
Article Snippet: RNA concentration was measured using a Qubit 4 fluorometer (Thermo Scientific, USA). .. Complementary DNA (cDNA) was synthesized using an MMLV reverse transcription kit (Protech, Taiwan) according to the manufacturer’s instructions. .. Each 20 μl reaction contained 200 ng RNA, 1 × reaction buffer, MMLV reverse transcriptase (20 units), RNase inhibitor, 1 mM of each dNTP, and oligo(dT) 18 , and was incubated at 42°C for 60 min. qRT-PCR was performed using the 2 × qPCRBIO SyGreen Blue Mix kit (PCR Biosystems Inc., USA).

Article Title: Immunosuppression of Tumor-Derived Factors Modulated Neutrophils in Upper Tract Urothelial Carcinoma Through Upregulation of Arginase-1 via ApoA1-STAT3 Axis
Article Snippet: .. The total RNA of the neutrophil was isolated using a GENEzol TM TriRNA Pure Kit (Geneaid, Taipei, Taiwan) according to the manufacturer’s protocol. cDNA was synthesized from 0.5 μg RNA using an MMLV Reverse Transcription Kit (Protech Technology Enterprise, Taipei, Taiwan). ..

Article Title: Immunosuppression of Tumor-Derived Factors Modulated Neutrophils in Upper Tract Urothelial Carcinoma Through Upregulation of Arginase-1 via ApoA1-STAT3 Axis.
Article Snippet: .. The total RNA of the neutrophil was isolated using a GENEzolTM TriRNA Pure Kit (Geneaid, Taipei, Taiwan) according to the manufacturer’s protocol. cDNA was synthesized from 0.5 μg RNA using an MMLV Reverse Transcription Kit (Protech Technology Enterprise, Taipei, Taiwan). ..

Synthesized:

Article Title: Polystyrene nanoplastics disrupt the intestinal microenvironment by altering bacteria-host interactions through extracellular vesicle-delivered microRNAs.
Article Snippet: Total RNA was extracted from renal tissues and cells using TRIzol® (Ambion,Waltham,MA, USA) following themanufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 2μg of total RNA using the MMLV Reverse Transcription Kit (Protech, Taipei, Taiwan). .. Quantitative PCR (qPCR) was performed using the Fast SYBRTM Green Master Mix (4385612, Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to themanufacturer’s protocol. mRNA expression levels were analyzed using real-time qPCR (StepOne Real-Time PCR System, USA).

Article Title: Polystyrene nanoplastics disrupt the intestinal microenvironment by altering bacteria-host interactions through extracellular vesicle-delivered microRNAs
Article Snippet: Total RNA was extracted from renal tissues and cells using TRIzol® (Ambion, Waltham, MA, USA) following the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 2 μg of total RNA using the MMLV Reverse Transcription Kit (Protech, Taipei, Taiwan). .. Quantitative PCR (qPCR) was performed using the Fast SYBRTM Green Master Mix (4385612, Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol. mRNA expression levels were analyzed using real-time qPCR (StepOne Real-Time PCR System, USA).

Article Title: Antiviral effect of lactoferrin against infectious bronchitis virus (IBV) are associated with alterations in the gastrointestinal microbiome
Article Snippet: RNA concentration was measured using a Qubit 4 fluorometer (Thermo Scientific, USA). .. Complementary DNA (cDNA) was synthesized using an MMLV reverse transcription kit (Protech, Taiwan) according to the manufacturer’s instructions. .. Each 20 μl reaction contained 200 ng RNA, 1 × reaction buffer, MMLV reverse transcriptase (20 units), RNase inhibitor, 1 mM of each dNTP, and oligo(dT) 18 , and was incubated at 42°C for 60 min. qRT-PCR was performed using the 2 × qPCRBIO SyGreen Blue Mix kit (PCR Biosystems Inc., USA).

Article Title: Immunosuppression of Tumor-Derived Factors Modulated Neutrophils in Upper Tract Urothelial Carcinoma Through Upregulation of Arginase-1 via ApoA1-STAT3 Axis
Article Snippet: .. The total RNA of the neutrophil was isolated using a GENEzol TM TriRNA Pure Kit (Geneaid, Taipei, Taiwan) according to the manufacturer’s protocol. cDNA was synthesized from 0.5 μg RNA using an MMLV Reverse Transcription Kit (Protech Technology Enterprise, Taipei, Taiwan). ..

Article Title: Immunosuppression of Tumor-Derived Factors Modulated Neutrophils in Upper Tract Urothelial Carcinoma Through Upregulation of Arginase-1 via ApoA1-STAT3 Axis.
Article Snippet: .. The total RNA of the neutrophil was isolated using a GENEzolTM TriRNA Pure Kit (Geneaid, Taipei, Taiwan) according to the manufacturer’s protocol. cDNA was synthesized from 0.5 μg RNA using an MMLV Reverse Transcription Kit (Protech Technology Enterprise, Taipei, Taiwan). ..

Isolation:

Article Title: Chronic sleep deprivation is associated with delayed puberty onset in rats, activation of proinflammatory cytokines and gut dysbiosis
Article Snippet: The RNA was extracted by using the RNeasy Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocol. .. Following RNA isolation, one μg of RNA was used for reverse transcription to cDNA by using the MMLV Reverse Transcription Kit (Protech Technology Enterprise, Co., Ltd., Taipei, Taiwan). cDNA was used to quantify the transcript levels on the Smart Quant Green Master Mix system (Protech Technology Enterprise, Co., Ltd., Taipei, Taiwan). ..

Article Title: Immunosuppression of Tumor-Derived Factors Modulated Neutrophils in Upper Tract Urothelial Carcinoma Through Upregulation of Arginase-1 via ApoA1-STAT3 Axis
Article Snippet: .. The total RNA of the neutrophil was isolated using a GENEzol TM TriRNA Pure Kit (Geneaid, Taipei, Taiwan) according to the manufacturer’s protocol. cDNA was synthesized from 0.5 μg RNA using an MMLV Reverse Transcription Kit (Protech Technology Enterprise, Taipei, Taiwan). ..

Article Title: Immunosuppression of Tumor-Derived Factors Modulated Neutrophils in Upper Tract Urothelial Carcinoma Through Upregulation of Arginase-1 via ApoA1-STAT3 Axis.
Article Snippet: .. The total RNA of the neutrophil was isolated using a GENEzolTM TriRNA Pure Kit (Geneaid, Taipei, Taiwan) according to the manufacturer’s protocol. cDNA was synthesized from 0.5 μg RNA using an MMLV Reverse Transcription Kit (Protech Technology Enterprise, Taipei, Taiwan). ..



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